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1.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 60: e198402, 2023. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1417480

ABSTRACT

The use of antimicrobials as growth promoters and disease prevention is being constantly reduced in several animal production systems, including in the swine industry. Therefore, this study aimed to evaluate the effectiveness of using acidifiers to control Salmonella Typhimurium in 65-day-old pigs by detecting the pathogen in organs at euthanasia. For this, 24 piglets were divided into two experimental groups consisting of 12 piglets each. An untreated control group (G1) and a treatment group (G2) received a liquid organic acidifier in the drinking water for 10 days (D-5 to D5). Five days after the start of treatment (D0), all piglets were challenged with 106 CFU of Salmonella Typhimurium and assessed for 12 days (D12). Every three days (D3, D6, D9, and D12), three animals from each experimental group were euthanized and then submitted for necropsy. Samples from the intestines (ileum, cecum, mesenteric lymph nodes, and ileocolic lymph nodes), liver, spleen, and lungs were collected to isolate Salmonella. The results show that, numerically, Salmonella isolation in the organs of G2 was lower than in G1 and that the number of positive cecum samples in G1 (66.7%; 8/12) was statistically different from the number of positive models in G2 (16.7%; 2/12), with a reduction of 28.6% of the total cecum positive samples in the treated group compared to the control. Therefore, it was observed that the liquid organic acidifier product could reduce the colonization of organs by Salmonella Typhimurium. (AU)


Subject(s)
Animals , Salmonella Infections/prevention & control , Swine/physiology , Organic Acids/analysis , Salmonella typhimurium/drug effects
2.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 60: e198402, 2023. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1419067

ABSTRACT

The use of antimicrobials as growth promoters and disease prevention is being constantly reduced in several animal production systems, including in the swine industry. Therefore, this study aimed to evaluate the effectiveness of using acidifiers to control Salmonella Typhimurium in 65-day-old pigs by detecting the pathogen in organs at euthanasia. For this, 24 piglets were divided into two experimental groups consisting of 12 piglets each. An untreated control group (G1) and a treatment group (G2) received a liquid organic acidifier in the drinking water for 10 days (D-5 to D5). Five days after the start of treatment (D0), all piglets were challenged with 106 CFU of Salmonella Typhimurium and assessed for 12 days (D12). Every three days (D3, D6, D9, and D12), three animals from each experimental group were euthanized and then submitted for necropsy. Samples from the intestines (ileum, cecum, mesenteric lymph nodes, and ileocolic lymph nodes), liver, spleen, and lungs were collected to isolate Salmonella. The results show that, numerically, Salmonellaisolation in the organs of G2 was lower than in G1 and that the number of positive cecum samples in G1 (66.7%; 8/12) was statistically different from the number of positive models in G2 (16.7%; 2/12), with a reduction of 28.6% of the total cecum positive samples in the treated group compared to the control. Therefore, it was observed that the liquid organic acidifier product could reduce the colonization of organs by Salmonella Typhimurium.(AU)


O uso de antimicrobianos como promotores de crescimento e prevenção de doenças vem sendo constantemente reduzido em diversos sistemas de produção animal, inclusive na suinocultura. Portanto, o objetivo do presente estudo foi avaliar a eficácia do uso de acidificantes no controle de Salmonella Typhimurium em suínos de 65 dias de idade, detectando o patógeno em órgãos após a eutanásia. Para isso, 24 leitões foram divididos em dois grupos experimentais constituídos por 12 leitões cada. Um grupo controle não tratado (G1) e um grupo de tratamento (G2) que recebeu um acidificante orgânico líquido na água de beber por 10 dias (D-5 a D5). Cinco dias após o início do tratamento (D0), todos os animais foram inoculados oralmente com 106 UFC de Salmonella Typhimurium e avaliados por 12 dias (D12). A cada três dias (D3, D6, D9 e D12), três leitões de cada grupo experimental foram eutanasiados e posteriormente submetidos à necropsia. Amostras de intestino (íleo, ceco, linfonodos mesentéricos e linfonodos ileocólicos), fígado, baço e pulmões foram coletadas para o isolamento de Salmonella. Os resultados mostram que, numericamente, o isolamento de Salmonella nos órgãos do G2 foi inferior ao G1, e que o número de amostras positivas de ceco no G1 (66,7%; 8/12) foi estatisticamente diferente do número de amostras positivas no G2 (16,7%; 2/12), com redução de 28,6% do total de amostras positivas de ceco no grupo tratado em relação ao controle. Portanto, observou-se que o ácido orgânico líquido foi capaz de reduzir a colonização de órgãos por Salmonella Typhimurium.(AU)


Subject(s)
Animals , Salmonella typhimurium/drug effects , Swine/physiology , Organic Acids/adverse effects , Salmonella Infections, Animal/drug therapy , Virus Shedding
3.
Arq. Inst. Biol ; 88: e00212020, 2021.
Article in English | LILACS, VETINDEX | ID: biblio-1349023

ABSTRACT

The consumption of meat and meat products can pose consumers into risk due to the presence of biological hazards that can cause foodborne diseases. Thus, this study aimed to compare the microbiological quality of illegal and inspected salami sold in the state of São Paulo, Brazil. For this purpose, 80 salami samples (40 illegal and 40 inspected) were purchased and their microbiological quality was assessed according to the protocol established by the Brazilian Ministry of Health. All samples were considered as acceptable for consumption according to the Brazilian law. However, the samples of illegal salami were significantly higher contaminated with bacteria belonging to the genus Staphylococcus (p = 0.002) and had a higher trend to be contaminated with total coliforms (p = 0.08) and thermotolerant ones (p = 0.07) compared to inspected salami. Salmonella spp. and coagulase-positive Staphylococcus were not detected. In conclusion, although all samples were considered as safe for consumption, illegal salami had a worse microbiological quality when compared to inspected ones.


Subject(s)
Humans , Animals , Staphylococcus/pathogenicity , Meat/microbiology , Meat Products , Salmonella , Bacteria , Health Surveillance , Food Quality , Public Health , Commerce , Food Safety , Foodborne Diseases
4.
Ciênc. rural (Online) ; 49(4): 20180694, 2019.
Article in English | LILACS | ID: biblio-1045339

ABSTRACT

ABSTRACT: Family Tayassuidae in the suborder Suina include two species of peccaries in Brazil: the white-lipped peccary (Tayassu pecari) and the collared peccary (Pecari tajacu). These animals share common pathogens with domestic swine (Sus scrofa); however, their role as potential carrier remains unclear. This study focused on detecting the prevalence of influenza A antibodies in Tayassu pecari and Pecari tajacu from commercial rearing farms from two states in Brazil. A set of 50 blood samples from Pecari tajacu and 55 from Tayassu pecari were analyzed using a commercial indirect ELISA in order to investigate anti influenza A antibodies. Pecari tajacu samples presented 22% (11/50) of seropositivity for the virus. Serological surveillance is an important tool to identify the presence and the spread of the influenza virus in feral pigs.


RESUMO: A família Tayassuidae pertencente a subordem Suina e compreende duas espécies presentes no Brasil: Queixada (Tayassu pecari) e o Caititu (Pecari tajacu). Ambas as espécies compartilham patógenos com o suíno doméstico (Sus scrofa), entretanto o papel destes animais como carreadores destas infecções permanece indefinido. O presente estudo teve como objetivo detectar a ocorrência de anticorpos contra vírus influenza A em amostras de soro de rebanhos comerciais de queixada e caititu, provenientes de dois estados do Brasil. Um total de 50 amostras de soro de Pecari tajacu e 55 amostras de Tayassu pecari foram testadas por meio de ELISA, sendo que 22% (11/50) das amostras de Pecari tajacu foram soropositivas para o agente. Estudos de vigilância sorológica são importantes para identificar a presença e a disseminação do vírus influenza em suínos selvagens.

5.
Arq. Inst. Biol ; 84: e0322016, 2017.
Article in English | LILACS, VETINDEX | ID: biblio-887853

ABSTRACT

Swine can be infected by the bovine viral diarrhea virus (BVDV) under natural conditions. For this reason, further information and divulgation are needed regarding the pathogenicity of this virus in swine. This infection is practically unknown in the realm of pig farming, and, as it shares some similarities with the classical swine fever virus (CSFV), its diagnosis becomes a challenge for official sanitary programs. Studies have shown the absence of clinical signs in piglets and reproductive problems in sows due to BVDV infections. There is little research on the prevalence, risk factors, preventive measures and control of BVDV in pigs around the world. And in Brazil, the data is practically non-existent. At the time of diagnosis, comparing the most efficient laboratory tests such as virus neutralization, ELISA, RT-PCR, and immunofluorescence so as to minimize the risk of cross serological reactions when dealing with a persistent or transient infection, can be an important tool. Moreover, the practical implications for CSFV eradication programs are a main reason for the development of further research against this infection. Therefore, this paper aims to review various aspects of BVDV infection in pigs, and how this information can be important for Brazilian herds.(AU)


O suíno pode ser infectado pelo vírus da diarreia viral bovina (BVDV) em condições naturais, por isso são necessárias maiores informações e mais divulgação sobre a ação deste vírus nos suínos. Esta infecção é praticamente desconhecida na suinocultura e, devido a algumas semelhanças com vírus da peste suína clássica (VPSC), torna-se um desafio para os programas sanitários oficiais. Estudos revelam a ausência de sinais clínicos em leitões ao mesmo tempo em que evidenciam problemas reprodutivos em porcas devido à infecção do BVDV. Poucas são as pesquisas sobre a prevalência, fatores de riscos, medidas de prevenção e controle do BVDV em suínos no mundo e, no Brasil, os dados são praticamente inexistentes. No diagnóstico, comparar os exames laboratoriais mais eficientes como a virusneutralização, ELISA, RT-PCR e imunofluorescência, diante de uma infecção persistente ou transitória, e assim minimizar o risco de reações sorológicas cruzadas pode ser uma ferramenta fundamental. Ademais, as implicações práticas em programas de erradicação da PSC são um grande motivo para o desenvolvimento de mais pesquisas frente a esta infecção. Portanto, este trabalho pretende revisar diversos aspectos da infecção do BVDV em suínos evidenciando o quanto essa situação pode ser importante para os rebanhos brasileiros.(AU)


Subject(s)
Animals , Swine , Diarrhea Virus 1, Bovine Viral , Food Safety , Health Surveillance , Brazil
6.
Ciênc. rural ; 46(9): 1607-1612, tab
Article in English | LILACS | ID: lil-787415

ABSTRACT

ABSTRACT: This study investigated the occurrence of antibodies for BVDV-1 in swine herds located in the region of Mossoró city of the state of Rio Grande do Norte, Brazil. A sample size of 412 animals was estimated assuming unknown prevalence (set at 50%). Virus neutralization assay was used to the detect the presence of antibodies for BVDV-1 and the results found were analysed using multivariable logistic regression model. The obtained prevalence was 4% at animal level and 45% at the animal and herd level. The titers were highly variable between animals and within farms. The multivariable logistic regression analysis showed an association between being housed outside and exposure to BVDV-1 (OR=0.24, 95% CI:0.06, 0.96, P=0.04). Highly correlated data and low prevalence of antibodies at the animal level resulted in insufficient power to detect significant differences with other selected risk factors. In conclusion, the prevalence is within the range reported for other countries.


RESUMO: Este estudo investigou a ocorrência de anticorpos contra o BVDV-1 em rebanhos suínos localizados na região da cidade de Mossoró do Estado do Rio Grande do Norte, Brasil. Um tamanho de amostra de 412 animais foi estimado assumindo uma prevalência desconhecida (fixada em 50%). O teste de virusneutralização foi utilizado para detectar a presença de anticorpos ao BVDV-1 e os resultados obtidos foram analisados usando o modelo de regressão logística multivariada. A prevalência obtida foi de 4% em nível animal e de 45% dos rebanhos. Os títulos foram muito variáveis entre os animais e dentro de fazendas. A análise de regressão logística multivariada apontou associação entre animais criados soltos e a exposição ao BVDV-1 (OR=0,24; 95% IC: 0,06; 0,96; P=0.04). A alta correlação entre os dados junto com a baixa prevalência de anticorpos a nível animal pode ter sido insuficiente para que as diferenças reais fossem detectadas. Em conclusão, a prevalência está dentro do intervalo referido em outros países.

7.
Rev. bras. ciênc. vet ; 21(1): 64-66, 2014. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1491551

ABSTRACT

Este trabalho objetivou avaliar a quantidade de água resultante do descongelamento de carcaças de aves oriundas de quatro abatedouros de aves no Estado de São Paulo, sob Serviço de Inspeção Estadual, através do método Drip Test, durante os anos de 2011 e 2012. Foram utilizadas 45 amostras, sendo que cada uma dessas era composta por seis carcaças de frango congeladas, determinando um total de 270 carcaças de aves utilizadas. Durante a realização do estudo, apenas 4,44% (2/45) das amostras apresentaram valores acima do limite aceitável determinado pelo Ministério da Agricultura, Pecuária e Abastecimento (MAPA), sendo que essas pertenciam a abatedouros distintos, porém não foi observada diferença significativa entre todos os estabelecimentos avaliados. Pode-se concluir que 4,44% das amostras do monitoramento apresentaram-se fora do padrão legal, oriundas de falhas tecnológicas e que resultam na insatisfação dos consumidores. É necessário um monitoramento de rotina, com colheita de amostras prontas para a comercialização sem prévio aviso, como prática a salvaguardar os interesses dos consumidores e à coibição de fraudes intencionais, principalmente naqueles estabelecimentos que evidenciem valores frequentemente elevados.


This study aimed to evaluate the amount of water resulting from poultry carcasses from four slaughterhouses at the São Paulo State, Brazil, through “Drip Test” method, during the years 2011 and 2012. It was used 45 samples, which were consisted of 6 chickenfrozen carcasses, totalizing 270 chickens. During the study, 4.44% (2/45) of the samples had values above the acceptable limit by Ministry of Agriculture, Livestock and Supply (MAPA), and were from two different establishments, but was not observed significant difference among all the establishments. It was concluded that some samples were not in concordance with legal standards, arising from technological failures that contributes to cause consumer dissatisfaction. Is necessary a routine monitoring system, utilizing samples ready for commercialization without previously notice, to safeguard the consumer’s interests and avoid intentional fraud, especially in those establishments that commonly presents high values.


Subject(s)
Animals , Frozen Foods , Chickens , Food Inspection , Poultry Products , Sanitary Inspection
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